array(1) { [0]=> object(AppBundle\Entity\geneTOpubmed)#305 (6) { ["id":"AppBundle\Entity\geneTOpubmed":private]=> int(215) ["geneNihId":"AppBundle\Entity\geneTOpubmed":private]=> int(34) ["pubmedNihId":"AppBundle\Entity\geneTOpubmed":private]=> int(3035565) ["title":"AppBundle\Entity\geneTOpubmed":private]=> string(116) "Nucleotide sequence of medium-chain acyl-CoA dehydrogenase mRNA and its expression in enzyme-deficient human tissue." ["urltitle":"AppBundle\Entity\geneTOpubmed":private]=> string(115) "nucleotide-sequence-of-medium-chain-acyl-coa-dehydrogenase-mrna-and-its-expression-in-enzyme-deficient-human-tissue" ["content":"AppBundle\Entity\geneTOpubmed":private]=> string(16686) " 3035565 1987 07 17 2016 10 19
0027-8424 84 12 1987 Jun Proceedings of the National Academy of Sciences of the United States of America Proc. Natl. Acad. Sci. U.S.A. Nucleotide sequence of medium-chain acyl-CoA dehydrogenase mRNA and its expression in enzyme-deficient human tissue. 4068-72 Medium-chain acyl-CoA dehydrogenase (MCAD; acyl-CoA: (acceptor) 2,3-oxidoreductase, EC 1.3.99.3) is one of three similar enzymes that catalyze the initial step of fatty acid beta-oxidation. Definition of the primary structure of MCAD and the tissue distribution of its mRNA is of biochemical and clinical importance because of the recent recognition of inherited MCAD deficiency in humans. The MCAD mRNA nucleotide sequence was determined from two overlapping cDNA clones isolated from human liver and placental cDNA libraries, respectively. The MCAD mRNA includes a 1263-base-pair coding region and a 738-base-pair 3'-nontranslated region. A partial amino acid sequence (137 residues) determined on peptides derived from MCAD purified from porcine liver confirmed the identity of the cDNA clone. Comparison of the amino acid sequence predicted from the human MCAD cDNA with the partial protein sequence of the porcine MCAD revealed a high degree (88%) of interspecies sequence identity. RNA blot analysis shows that MCAD mRNA is expressed in a variety of rat (2.2 kilobases) and human (2.4 kilobases) tissues. Blot hybridization of RNA prepared from cultured skin fibroblasts from a patient with MCAD deficiency disclosed that mRNA was present and of similar size to MCAD mRNA derived from control fibroblasts. The isolation and characterization of MCAD cDNA is an important step in the definition of the defect underlying MCAD deficiency and in understanding its metabolic consequences. Kelly D P DP Kim J J JJ Billadello J J JJ Hainline B E BE Chu T W TW Strauss A W AW eng GENBANK M16827 AM-20407 AM NIADDK NIH HHS United States GM 29076 GM NIGMS NIH HHS United States HL-17646 HL NHLBI NIH HHS United States Journal Article Research Support, U.S. Gov't, P.H.S.
United States Proc Natl Acad Sci U S A 7505876 0027-8424 0 RNA, Messenger 9007-49-2 DNA EC 1.3.- Acyl-CoA Dehydrogenases EC 1.3.8.7 Acyl-CoA Dehydrogenase EC 3.1.21.- DNA Restriction Enzymes IM J Biol Chem. 1985 Jan 25;260(2):1311-25 3968063 N Engl J Med. 1984 Nov 8;311(19):1232-6 6493275 J Pediatr. 1985 Jun;106(6):918-21 3998948 Pediatr Res. 1985 Jul;19(7):666-71 4022672 Pediatr Res. 1985 Jul;19(7):671-6 4022673 J Biol Chem. 1985 Dec 5;260(28):14988-92 4066661 EMBO J. 1986 Jun;5(6):1335-42 3015599 Pediatr Res. 1986 Sep;20(9):843-7 3748657 Proc Natl Acad Sci U S A. 1986 Sep;83(17):6543-7 3462713 Adv Enzymol Relat Subj Biochem. 1962;24:291-358 13884182 Biophys J. 1967 Mar;7(2):121-35 6048867 J Biol Chem. 1975 May 10;250(9):3476-86 1168197 Arch Biochem Biophys. 1976 Dec;177(2):402-14 1015826 Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 271968 Biochemistry. 1979 Jan 23;18(2):331-7 570409 J Biol Chem. 1982 Jan 25;257(2):971-8 6798036 Arch Biochem Biophys. 1982 Jan;213(1):155-62 7059177 J Biochem. 1981 Dec;90(6):1739-50 7334008 Science. 1983 Jul 1;221(4605):73-5 6857268 J Biol Chem. 1983 Jun 25;258(12):7738-45 6863262 Anal Biochem. 1983 Jul 1;132(1):6-13 6312838 Pediatr Res. 1983 Nov;17(11):877-84 6646897 Nucleic Acids Res. 1984 Jan 25;12(2):857-72 6694911 Acta Paediatr Scand. 1983 Nov;72(6):943-9 6673498 J Mol Biol. 1984 Apr 15;174(3):483-96 6546954 Annu Rev Biochem. 1984;53:595-623 6383201 Biochem Med. 1985 Feb;33(1):38-44 3994700 Acyl-CoA Dehydrogenase Acyl-CoA Dehydrogenases deficiency genetics isolation & purification Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA metabolism DNA Restriction Enzymes Humans Mitochondria, Liver enzymology Protein Biosynthesis RNA, Messenger genetics Rats Skin enzymology Swine
1987 6 1 1987 6 1 0 1 1987 6 1 0 0 ppublish 3035565 PMC305023
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